404 error when trying and unable access the sub urls but the server name is running - apache

Question

I am deploying Flask app with httpd 2.4 by using mod_wsgi 4.5 version.
My servername sample.server.com is running fine but when i try with sub url like sample.server.com/app1, am encountering 404 error.
I also tried with localshost/app1 its is working, that means my flask app is tottaly fine but there is some configuration issue which i couldnt figure out.
I am providing with wsgi file and virtualhost config, i tried reading many articles and blogs but i couldnt find the solution.I would appreciate any help, Thanks
app.wsgi-->
activate_this = '/home/nmapi/venv/bin/activate_this.py'
with open(activate_this) as file_:
exec(file_.read(), dict(__file__=activate_this))
from werkzeug.wsgi import DispatcherMiddleware
import sys
import logging
print(sys.path)
logging.basicConfig(stream=sys.stderr)
sys.path.insert(0,"home/nmapi/project/dir")
from run import *
my vurtual host config is
#ServerName sample.server.com
WSGIApplicationGroup %{GLOBAL}
WSGIScriptAlias /test_wsgi
/home/nmapi/project/dir/datascience.wsgi
<Directory /home/nmapi/project/dir/>
#WSGIProcessGroup score
#AllowOverride All
Require all granted
#Allow from all
</Directory>
Alias /static /home/nmapi/project/dir/static
<Directory /home/nmapi/project/dir/static/>
WSGIProcessGroup score
#WSGIApplicationGroup %{GLOBAL}
#AllowOverride All
Require all granted
#Allow from all
</Directory>
ErrorLog logs/error.log
#ErrorLog /tmp/error.log
LogLevel debug
#CustomLog /tmp/access.log combined
CustomLog logs/access.log combined